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Advanced Biotech Miscellaneous

  1. How many standard λ-phage vectors carrying 20kb DNA fragments or YACs carrying 250 kb DNA fragments are theoretically required to constitute a complete C. elegans genomic library?
    1. 500 λ-phage vectors or 40 yeast clones
    2. 400 λ-phage vectors or 5000 yeast clones ​​
    3. 5000 λ-phage vectors or 400 yeast clones
    4. ​5 × 104 λ-phage vectors or 4000 yeast clones ​
Correct Option: C

Number of λ-phage vectors =
1.0 × 108
= 5 × 103
20 × 103

Number of yeast clones =
108
= 400
250 × 103



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