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Advanced Biotech Miscellaneous

  1. Somatic embryo from cotyledon explant would develop in the following sequential stages,
    1. cotyledonary → heart → globular → torpedo
    2. globular → torpedo → heart → cotyledonary
    3. globular → heart → torpedo → cotyledonary
    4. ​cotyledonary → globular → heart torpedo ​
Correct Option: C

In the globular stage, the embryo develops radial patterning through a series of cell divisions, with the outer layer of cells differentiating into the ‘protoderm.’ The globular embryo can be thought of as two layers of inner cells with distinct developmental fates; the apical layer will go on to produce cotyledons and shoot meristem, while the lower layer produces the hypocotyl and root meristem. Bilateral symmetry is apparent from the heart stage; provascular cells will also differentiate at this stage. In the subsequent torpedo and cotyledonary stages of embryogenesis, the embryo completes its growth by elongating and enlarging.



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