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A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled ?
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- 4 labeled; 6 unlabeled
- 4 labeled; 4 unlabeled
- 3 labeled; 5 unlabeled
- 3 labeled; 3 unlabeled
Correct Option: C
Let the DNA be the following line :
The cut portions are E.CoRI digestion site. So the partial digestion can generate 3 smaller fragments with label and 5 fragments without any level.
