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Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
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- the sequence context at the RB defines the direction of transfer
- the sequence context at the LB defines the direction of transfer
- the nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand
- the endonuclease activity of VirD2 protein allows nicking at RB
Correct Option: A
virulence genes are grouped genes for synthesis of opines and plant hormones are contained on the T-DNA; T-DNA is bordered by 25 bp direct repeats; only the TR border is required for transfer although the Ti border increased the efficiency when it is present; no sequences other that the borders are required for transfer.
