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Expression in poor amount and in inactive form of cDNA of a euharyotic protein in Escherichia coli using its expression vector is due to
(P) the absence of capping mechanism of mRNA
(Q) codon bias
(R) absence of polyadenylation
(S) absence of proper glycosylation
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- P, Q
- Q, R
- Q, S
- P, S
Correct Option: D
The process of 52 capping is vital to creating mature messenger RNA, which is then able to undergo translation. Capping ensures the messenger RNA’s stability while it undergoes translation in the process of protein synthesis. Glycosylation confers stability to the proteins and is also required for the folding of proteins. Absence of either of these mechanisms result in poor expression and inactivity.
