Direction: A recombinant SV40 virus delivers c-myc cDNA, which has a unique sal I site, into muscle cells. Southern analysis of Sal I digested total genomic DNA of the muscle cell using c-myc cDNA porbe generates a smear.
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The DNA smear obtained on Southern blot is due to
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- head to head concatamer of viral DNA
- head to tail concatamer of viral DNA
- tail to tail concatamer of viral DNA
- random integration of viral DNA
Correct Option: B
The replication intermediates of parvoviruses thus are concatemers of head-to-head or tail-to-tail structure. Surprisingly, in case of the novel human bocavirus, neither head-to-head nor tail-to-tail DNA sequences were detected in clinical isolates; in contrast head-to-tail DNA sequences were identified by PCR and sequencing. Thereby, the head-to-tail sequences were linked by a novel sequence which can be detected by southern blot.
