Tobacco leaf discs are transfected with Agrobacterium tumefaciens

Tobacco leaf discs are transfected with Agrobacterium tumefaciens strain containing binary vector (GUS as reporter gene) with selectable marker neo (kanamycin resistant gene) and then regenerated to plants. The plants are kanamycin resistant but leaf tissues are negative to GUS assay. The explanations are

1. The plants are transformed for both genes by GUS gene is turned off
2. The plants are transformed for only neo gene not the GUS gene
3. The plants are not transformed at all, but the development of kanamycin resistance is due to somaclonal variation
4. All of these

The plants are not transformed at all, but the development of kanamycin resistance is due to the somaclonal variation.