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The restriction endonuclease HaeIII recognizes the sequence GG ↓ CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice ?
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- SmaI (CC ↓ GGG)
- NotI(GC ↓ GGCCGC)
- SalmIII (GG ↓ CC)
- PstI (CTGCA ↓ G)
Correct Option: C
The plasmid contains a genetic sequence corresponding to the recognition site of a restriction endonuclease, such as HaeIII. After foreign DNA fragments, which have also been cut with the same or similar restriction endonuclease, have been inserted into host cell, the restriction endonuclease gene is expressed by applying heat, or by introducing a biomolecule, such as arabinose.
