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For the sequence of ds DNA given below, identify the set of primers required to amplity this DNA by PCR
3′ GACTCCA.............TACAACC 5′
CTGAGGT.................ATGTTGG 3′
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- 5′ GGTTGTA and 5′ GACTCCA
- 5′ CTGAGGT and 5′ CCCAACAT
- 5′ ACTCAGT and 5′ ATGTTGG
- None of these
Correct Option: A
The polymerase starts replication at the 3’ end of the primer which means 5’ end of the template and hence A is the correct option.
