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The culture fluids of 1000 to 5000 colonies of hybridoma are screened for monoclonal antibody by
P. Western blot analysis
Q. Antigen capture analysis
R. Northern blot analysis
S. Antibody capture analysis Choose the correct pair from the following :
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- P, Q
- Q, R
- R, S
- Q, S
Correct Option: A
Monoclonal antibodies bind to a single epitope within a target antigen. Western blot procedures allow for flexibility in choosing gel electrophoresis and blotting conditions, it is possible to modify buffers to retain enough higher order protein structure for detection by some antibodies. Thus can be brought to usage in screening for monoclonal antibodies.
