Biophysical Miscellaneous


Biophysical Miscellaneous

  1. The limiting factor in the production of large quantities of ethanol as bio-fuel is









  1. View Hint View Answer Discuss in Forum

    For ethanol commercial utilization we need high purity which leads to heavy downstream processing cost along with high percentage of ethanol cause dehydration to the microbial inoculums leading to toxicity to cell.

    Correct Option: D

    For ethanol commercial utilization we need high purity which leads to heavy downstream processing cost along with high percentage of ethanol cause dehydration to the microbial inoculums leading to toxicity to cell.


  1. Match the items in Group I with Group II. ​​
    Group I ​​
    P.​Circular dichroism ​​
    Q.​X-ray crystallography ​​
    R.​Freeze-drying ​​
    S.​Ultracentrifugation ​​
    Group II ​​
    1.​Concentration ​​
    2.​Sedimentation coefficient
    ​​3.​Secondary structure determination ​​
    4.​Tertiary structure determination









  1. View Hint View Answer Discuss in Forum

    Protein secondary structure can be determined by the Circular Dichromism where as XRD can be used for the Tertiary structure prediction of the protein. Freeze drying is an unit operation for the concentration increase in a solution of limited solute. Ultra centrifugation is the unit operation for sedimentation. ​

    Correct Option: D

    Protein secondary structure can be determined by the Circular Dichromism where as XRD can be used for the Tertiary structure prediction of the protein. Freeze drying is an unit operation for the concentration increase in a solution of limited solute. Ultra centrifugation is the unit operation for sedimentation. ​



  1. Match the properties in Group 1 with the downstream operation in Group 2 :
    Group 1 Group 2
    P. Size 1. Extraction
    ​​Q. Density ​2. Distillation
    R. Volatility 3. Filtration
    S. Solubility 4. Sedimentation
    ​​









  1. View Hint View Answer Discuss in Forum

    All the properties in column A are phenomena unit for downstream process in column B. ​

    Correct Option: A

    All the properties in column A are phenomena unit for downstream process in column B. ​


  1. Match the coefficients in group 1 with their corresponding downstream processing steps given in group 2 ​​
    Group 1 ​​
    ​​P.​Sedimentation coefficient​​ ​​
    Q.​Partition coefficient ​​
    R.​Rejection coefficient ​​
    S.​Activity coefficient ​​
    Group 2 ​​
    1.​Aqueous two phase extraction ​​
    2.​Ultrafiltration ​​
    3.​Dialysis
    ​​4.​Centrifugation









  1. View Hint View Answer Discuss in Forum

    Svedbarg Unit stands for sedimentation coefficient for centrifugation process. Partition coefficient for two layer aqueous extraction unit. Rejection coefficient is the unit for ultra filtration. Dialysis unit is activity coefficient.

    Correct Option: D

    Svedbarg Unit stands for sedimentation coefficient for centrifugation process. Partition coefficient for two layer aqueous extraction unit. Rejection coefficient is the unit for ultra filtration. Dialysis unit is activity coefficient.



  1. Match each parameter in group 1 with the appropriate measuring device in group 2 ​​
    Group 1 ​​
    P.​Pressure ​​
    Q.​Foam ​​
    R.​Turbidity ​​
    S.​Flow rate ​​
    Group 2 ​​
    1.​Photometer ​​
    2.​Rotameter ​​
    3.​Diaphragm gauge ​​
    4.​Rubber sheathed electrode









  1. View Hint View Answer Discuss in Forum

    ⚈ The Diaphragm Pressure Gage uses the elastic deformation of a diaphragm (i.e. membrane) instead of a liquid level to measure the difference between an unknown pressure and a reference pressure. ​​
    ⚈ ​In an antifoam system; if the foam contacts a rubber sheathed electrode, an electric unit actuates a solenoid valve to allow the passage of a sterile antifoam agent into the fermentor.
    ⚈ A final widely used method for the determination of cell number is a turbidometric measurement or light scattering. This technique depends on the fact that as the number of cells in a solution increases, the solution becomes increasingly turbid (cloudy). The solution looks turbid because light passing through it is scattered by the microorganisms present and the turbidity is proportional to the number of microorganisms in the solution. The turbidity of a culture can be measured using a photometer or a spectrophotometer. Photometers, such as the Klett-Summerson device, use a red, green or blue filter providing a broad spectrum of light. ​​
    ⚈ A rotameter is a device that measures the flow rate of liquid or gas in a closed tube.

    Correct Option: A

    ⚈ The Diaphragm Pressure Gage uses the elastic deformation of a diaphragm (i.e. membrane) instead of a liquid level to measure the difference between an unknown pressure and a reference pressure. ​​
    ⚈ ​In an antifoam system; if the foam contacts a rubber sheathed electrode, an electric unit actuates a solenoid valve to allow the passage of a sterile antifoam agent into the fermentor.
    ⚈ A final widely used method for the determination of cell number is a turbidometric measurement or light scattering. This technique depends on the fact that as the number of cells in a solution increases, the solution becomes increasingly turbid (cloudy). The solution looks turbid because light passing through it is scattered by the microorganisms present and the turbidity is proportional to the number of microorganisms in the solution. The turbidity of a culture can be measured using a photometer or a spectrophotometer. Photometers, such as the Klett-Summerson device, use a red, green or blue filter providing a broad spectrum of light. ​​
    ⚈ A rotameter is a device that measures the flow rate of liquid or gas in a closed tube.