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Biophysical Miscellaneous

  1. Match the items in Group I with Group II. ​​
    Group I ​​
    P.​Circular dichroism ​​
    Q.​X-ray crystallography ​​
    R.​Freeze-drying ​​
    S.​Ultracentrifugation ​​
    Group II ​​
    1.​Concentration ​​
    2.​Sedimentation coefficient
    ​​3.​Secondary structure determination ​​
    4.​Tertiary structure determination
    1. P - 4 , Q - 1 , R - 2 , S - 3
    2. P - 1 , Q - 4 , R - 3 , S - 2
    3. P - 2 , Q - 3 , R - 4 , S - 1
    4. P - 3 , Q - 4 , R - 1 , S - 2
Correct Option: D

Protein secondary structure can be determined by the Circular Dichromism where as XRD can be used for the Tertiary structure prediction of the protein. Freeze drying is an unit operation for the concentration increase in a solution of limited solute. Ultra centrifugation is the unit operation for sedimentation. ​



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