Biotechnology and its Applications


  1. Consider the following statements (A-D) about organic farming:
    (A) Utilizes genetically modified crops like Bt cotton
    (B) Uses only naturally produced inputs like compost
    (C) Does not use pesticides and urea
    (D) Produces vegetables rich in vitamins and minerals
    Which of the above statements are correct?









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    Organic farming is the form of agriculture that relies on the techniques like crop rotation, green manure, compost and biological pest control.

    Correct Option: C

    Organic farming is the form of agriculture that relies on the techniques like crop rotation, green manure, compost and biological pest control.


  1. The genetically-modified (GM) brinjal in India has been developed for:









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    The genetically modified brinjal in India has been developed for insect resistance. Bt brinjal is a transgenic brinjal that is developed by inserting a crystal gene from the Bacillus thuringiensis into the brinjal’s genome. This process of insertion is accomplished using Agrobacterium mediated recombination.

    Correct Option: A

    The genetically modified brinjal in India has been developed for insect resistance. Bt brinjal is a transgenic brinjal that is developed by inserting a crystal gene from the Bacillus thuringiensis into the brinjal’s genome. This process of insertion is accomplished using Agrobacterium mediated recombination.



  1. Bacillus thuringiensis forms protein crystals which contain insecticidal protein.









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    Bacillus thuringiensis produces a large amount of crystalline protein during sporulation. In the cell toxins are formed along with the spore and are referred to as parasporal body. The bacteria are capable of entering the insect’s blood and using the host insect to reproduce. The proteins from ingested spores are activated by gut, high pH and the polypeptide toxins destroy gut epithelial cells and kill the pest.

    Correct Option: A

    Bacillus thuringiensis produces a large amount of crystalline protein during sporulation. In the cell toxins are formed along with the spore and are referred to as parasporal body. The bacteria are capable of entering the insect’s blood and using the host insect to reproduce. The proteins from ingested spores are activated by gut, high pH and the polypeptide toxins destroy gut epithelial cells and kill the pest.


  1. Some of the characteristics of Bt cotton are:









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    Bt toxin genes were isolated from Bacillus thuringiensis and incorporated into cotton plant to form a genetically modified crop called Bt cotton. Bt cotton has high yield and resistance to bollworms.

    Correct Option: D

    Bt toxin genes were isolated from Bacillus thuringiensis and incorporated into cotton plant to form a genetically modified crop called Bt cotton. Bt cotton has high yield and resistance to bollworms.



  1. Silencing of mRNA has been used in producing transgenic plants resistant to:









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    In this technique nematode specific genes are introduced in the host plant in such a way that it produces both sense and antisense RNA. The two RNA’s being complementary to each other from a double stranded RNA (dsRNA) which is also called interfering RNA responsible for initiating RNA interference (RNA i). This (dsRNA) bind to and prevent translation of specific mRNA of nematode (gene silencing). Thus transgenic plants based on RNAi technology are resistant to nematode.

    Correct Option: B

    In this technique nematode specific genes are introduced in the host plant in such a way that it produces both sense and antisense RNA. The two RNA’s being complementary to each other from a double stranded RNA (dsRNA) which is also called interfering RNA responsible for initiating RNA interference (RNA i). This (dsRNA) bind to and prevent translation of specific mRNA of nematode (gene silencing). Thus transgenic plants based on RNAi technology are resistant to nematode.