Reverse genetics is an approach to discover the function of a gene by analyzing the phenotypic effects of specific gene sequences obtained by DNA sequencing. This investigative process proceeds in the opposite direction of so-called forward genetic screens ofclassical genetics. Simply put, while forward genetics seeks to find the genetic basis of a phenotype or trait, reverse genetics seeks to find what phenotypes arise as a result of particular genetic sequences.

Correct Option: A

Reverse genetics is an approach to discover the function of a gene by analyzing the phenotypic effects of specific gene sequences obtained by DNA sequencing. This investigative process proceeds in the opposite direction of so-called forward genetic screens ofclassical genetics. Simply put, while forward genetics seeks to find the genetic basis of a phenotype or trait, reverse genetics seeks to find what phenotypes arise as a result of particular genetic sequences.

β-Lactoglobulin is the major whey protein of cow and sheep’s milk (~3 g/l), and is also present in many other mammalian species; a notable exception being humans. Its structure, properties and biological role have been reviewed many times.

Correct Option: B

β-Lactoglobulin is the major whey protein of cow and sheep’s milk (~3 g/l), and is also present in many other mammalian species; a notable exception being humans. Its structure, properties and biological role have been reviewed many times.

Each groove in DNA is lined by potential hydrogen-bond donor and acceptor atoms that enable specific interactions with proteins (seeFigure 27.7). In the minor groove, N-3 of adenine or guanine and O-2 of thymine or cytosine can serve as hydrogen acceptors, and the amino group attached to C-2 of guanine can be a hydrogen donor. In the major groove, N-7 of guanine or adenine is a potential acceptor, as are O-4 of thymine and O-6 of guanine. The amino groups attached to C-6 of adenine and C-4 of cytosine can serve as hydrogen donors. 

Correct Option: C

Each groove in DNA is lined by potential hydrogen-bond donor and acceptor atoms that enable specific interactions with proteins (seeFigure 27.7). In the minor groove, N-3 of adenine or guanine and O-2 of thymine or cytosine can serve as hydrogen acceptors, and the amino group attached to C-2 of guanine can be a hydrogen donor. In the major groove, N-7 of guanine or adenine is a potential acceptor, as are O-4 of thymine and O-6 of guanine. The amino groups attached to C-6 of adenine and C-4 of cytosine can serve as hydrogen donors. 

Degeneracy produces the ultimate amino acid combination for protein expression.

Correct Option: C

Degeneracy produces the ultimate amino acid combination for protein expression.

Heterologous protein expression depends upon good codon biasing, transcriptional efficiency and optimum copy number production of the expression vector system.

Correct Option: D

Heterologous protein expression depends upon good codon biasing, transcriptional efficiency and optimum copy number production of the expression vector system.