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An analysis of chromosomal DNA using the Southern hybridization technique does not use:
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- Electrophoresis
- Blotting
- Autoradiography
- PCR
Correct Option: D
PCR is a technique for enzymatically replicating DNA without using a living organism such as E. coli or Yeast. It is commonly used in medical and biological research labs for a variety of tasks like detection of hereditary diseases, identification of genetic fingerprints etc.