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Biotechnology Principles and Processes

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  1. During the process of isolation of DNA, chilled ethanol is added to
    1. Remove proteins such as histones
    2. Precipitate DNA
    3. Break open the cell to release DNA
    4. Facilitate action of restriction enzymes
Correct Option: B

Ethanol is non-polar molecule while DNA is a polar molecule, it has a net negative charge due to the phosphate groups attached to it. When DNA is placed into a solution of 100% ethanol, it becomes insoluble and precipitates out of solution.


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