Correct Option: B

Point accepted mutation (PAM) matrix is used for species having deletion or insertion at any particular point in the nucleotide sequence. Block substitution matrix (BLOSUM) is used for species whose sequences have undergone substitution. PAM 40 means that 40 % of the sequences are mutated and 60% are conserved while BLOSUM 80 means that 80% of the sequences are conserved and rest 20% are mutated. So, PAM 40 and BLOSUM 80 can be used for less divergent or closely related species for performing the alignment.