Correct Option: D

The Expect value (E) is a parameter that describes the number of hits one can "expect" to see by chance when searching a database of a particular size. It decreases exponentially as the Score (S) of the match increases. Essentially, the E value describes the random background noise. Virtually identical short alignments have relatively high E values. This is because the calculation of the E value takes into account the length of the query sequence and also depends upon the number of sequences in the database. These high E values make sense because shorter sequences have a higher probability of occurring in the database purely by chance.
Thus the E value does not depend on the probability from a normal distribution.