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Biochemical Miscellaneous

  1. ​In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is  20 gl–1 (DCW), the specific growth rate (µ) is 0.4h–1 and substrate uptake rate (ν) is 16 gl–1h–1. The cell yield coefficient Yx/s will be ​
    1. ​0.32 ​
    2. 0.64 ​
    3. 0.80 ​
    4. 0.50
Correct Option: D

Yield coefficient = mass of new cells formed / mass of substrate consumed ​​
New cells mass formed = cell density x specific growth rate = 20 × 0.4 = 8
​​Substrate consumed = 16 g/l/h ​​

Yield coefficient =
8
= 0.5
16



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