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Match the downstream processes in Group I with the products in Group II.
Group I Group II P. Solvent extraction 1. Lactic acid Q. Protein-A linked affinity 2. Penicillin chromatography R. Extractive distillation 3. Monoclonal antibody S. Salting out 4. Lipase
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- P-2 , Q-3 , R-1 , S-4
- P-4 , Q-1 , R-2 , S-3
- P-4 , Q-1 , R-3 , S-2
- P-2 , Q-4 , R-1 , S-3
Correct Option: A
Solvent extraction is a method for processing materials by using a solvent to separate the parts. It can be used for penicillin extraction.
Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. Each is designed for highly specific and efficient purification of proteins and related compounds like monoclonal antibody.
Extractive distillation is defined as distillation in the presence of a miscible, high boiling, relatively non-volatile component, and the solvent that forms no azeotrope with the other components in the mixture. It can be used for lactic acid recovery.
Salting out is a purification method that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength. Crude lipase can be purified by using salting out method.
