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A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 105 anchorage dependent cells per cm2, the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are
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- 1200 cm2 and 12 × 107 cellls
- 600 cm2 and 6 × 107 cells
- 600 cm2 and 8300 cells
- 1200 cm2 and 8300 cells
Correct Option: B
Given, Inner radius (r) = 10 cm
Length (l) = 20 cm
Length of spiral film (L)= 30 cm
Width (W) = 20 cm
Thus, the increase in the surface area after fitting of surface film = 30 * 20 = 600 cm2
Also, the film can support 105 anchorage dependent cells per cm2
Thus, the film will add = 105 cells/cm2 * 600 cm2 = 6 * 107 cells.
