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During the media preparation for cultivation of cells, insoluble precipitates of calcium phosphates are often formed. Identify which method can be adopted to avoid this problem?
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- Hold the pH at 5.6
- Hold the pH at 7.5
- Add calcium salt first and then phosphate source
- None the above
Correct Option: D
The equilibrium solubility of four calcium salts (calcium oxalate hydrate, calcium citrate tetrahydrate, calcium phosphate, calcium glycerophosphate) were determined at controlled pH values (7.5, 6.0, 4.5 and < or = 3.0) and in distilled water.
