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Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of
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- sodium alginate and dropping it in a solution of calcium nitrate
- calcium alginate and dropping it in a solution of sodium nitrate
- calcium alginate and dropping it in a solution of ammonium nitrate
- mannitol and dropping it in a solution of sodium nitrate
Correct Option: A
Synthetic seeds or artificial seeds are seed-like structures derived from somatic embryos under in vitro condition after encapsulation by hydrogen. These are tissue culture derived somatic embryos encased in a protective coating. Hydrated synthetic seeds are produced in those plant species where the somatic embryos are recalcitrant and sensitive to desiccation. Hydrated synthetic seeds are produced by encapsulating the somatic embryos in hydrogel capsules. The somatic embryos are mixed with sodium alginate (2 %) and the suspension is dropped into the calcium salts solution (200mM). The principle involved is when sodium alginate dropped into the calcium salt solutions it form round firm beads due to the ion exchange between Na+ in sodium alginate and Ca2+ in calcium slat solutions and sodium alginate form calcium alginate in 20-30 minutes. The calcium salt used can be calcium nitrate.