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To produce plants that are homozygous for all traits, the best choice is
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- Protoplast culture
- Cell suspension culture
- Anther and pollen culture
- Apical merisem culture
Correct Option: C
In anther culture, the anther swells and dehisces along its upper margin, lengthwise. This phenomenon helps to expose the pollen grain. Alternatively, huge amount of pollen grains can be isolated manually and can be cultured aseptically very easily. Therefore, pollen is more suitable material than egg cell for the production of haploid. The development and production of haploid plant in vitro is very important for the study of fundamental and applied aspects of genetics in the higher plants. Production of homozygous diploid by doubling the chromosome number of haploid in vitro makes a pure line in single step and such homozygous pure line is of great importance in plant breeding. Also it is easy to induce cell division in immature pollen cells in some species. By anther and pollen culture, haploids can be produced in large numbers very quickly.