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Advanced Biotech Miscellaneous

  1. To promote attachment and spreading of anchorage-dependent animal cells, the surface of the culture vessel needs to be coated with ​
    1. trypsin
    2. collagen
    3. pronase
    4. polyglycol
Correct Option: B

Type I collagen, a fibrous protein abundant in connective tissues including tendon, ligament, dermis and blood vessel, is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells and etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function. ​



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