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For amplificatio of GC rich sequences by polymerase chain reacstion, identify the reagent that binds and stabilizes AT sequences and destabilizes GC regions.
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- Tetramethyl ammonium chloride
- Betaine
- 7-deaza-2′-deoxyguanosine triphosphate
- Sodium dodecyl sulphate
Correct Option: B
Betaine improves the PCR amplification of GC-rich DNA sequences. Betaine reduces the formation of secondary structure caused by GC-rich regions and, therefore can be used to assist the amplification of GC rich sequences by PCR. It facilitates an isostabilizing effect on the differential thermal stability of AT and GC base pairs, which may result in a larger destabilizing effect on GC pairs and stabilzes AT rich sequence by binding with them and causing conformational change.
