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Advanced Biotech Miscellaneous

  1. Expression of hundreds of different genes in DNA microarray technology is monitored by using ​​
    1. Radioactive probe ​​
    2. Visible chromogenic probe
    3. UV absorbing probe
    4. ​Fluorescent probe ​
Correct Option: D

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles (10”12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti-sense RNA) sample (called target) under high-stringency conditions. Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target.



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