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  1. For their efficient translation, eubacterial mRNAs possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence (ASD) in the ribosomes. The correct statement is
    1. ASD is present in 5S rRNA
    2. ASD is present in 23S rRNA
    3. ASD is present in 16S rRNA
    4. ASD is formed by the interaction of the 16S rRNA with the 23S rRNA upon docking of the 50S subunit on the 30S subunit of the ribosomes
Correct Option: C

Shine-Dalgarno (SD) sequence is a ribonucleotide sequence for the formation of the preinitiation complex between a 30S ribosomal subunit and an mRNA molecule. It is a seven-nucleotide, pyrimidine-rich sequence near the 3? terminus of 16S (prokaryotic) or 18S eukaryote ribosomal RNA, found in many organisms, that forms base pairs with a complementary three- to seven-nucleotide, purine-rich sequence (the Shine-Dalgarno sequence) preceding the initiation codon in many mRNA molecules, thereby allowing the initiating ribosomal subunit to bind the mRNA so as to discriminate between AUG or GUG (i.e. initiation) triplets and to select the one at the beginning of a cistron. Anti Shine Dalgarno sequences are present in 16s rRNA molecules for the recognition of SD sequences in eubacterial mRNA.



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