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During transcription
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- DNA Gyrase introduces negative supercoils and DNA Topoisomerase I removes negative supercoils
- DNA Topoisomerase I introduces negative supercoils and DNA Gyrase removes negative supercoils
- both DNA Gyrase and DNA
Topoisomearse I introduce negative supercoils - both DNA Gyrase and DNA Topoisomearse I remove negative supercoils
Correct Option: A
DNA gyrase catalyse ATP dependant –ve supercoiling as these are topoisomerases which influence the topological state of DNA. It is type II topoisomerase which induce –ve supercoils (or relax +ve supercoils) by looping the template and cutting one of the double helices and then 2 ends are twisted for –ve supercoils. Type I topoisomerase unwinds –ve supercoils to control protein synthesis and facilitate DNA replication, and ease out problems due to unwinding of double helical forms.
