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  1. The viability of seeds is tested by
    1. Safranine
    2. 2, 6 dichlorophenol indophenols
    3. 2, 3, 5 triphenyl tetrazolium chloride
    4. DMSO
Correct Option: C

Dehydrogenase enzymes present in living tissue reduce the tetrazolium chloride to formazan, a reddish, water insoluble compound. This reaction occurs in or near living cells which are releasing hydrogen in respiration processes. Viable tissues produce a normal red-colour, weak living tissue produce an abnormal colour. Dead tissues do not stain, remaining usually white.



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