Correct Option: B

Rupture and fractionation do not usually occur in the water column in vessel/ tracheids during the ascent of sap because of cohesion and tension. The water molecules have a great mutual attraction to each other or tremendous cohesive power which is sometimes as much as 350 atmospheres. Thus, the transpiration pull develops a negative pressure in the uppermost xylem cells. It is transmitted from there into the xylem of stems, and then to the roots.