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A student wishes to study the cell structure under a light microscope having 10X eyepice and 45X objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?
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- Blue
- Green
- Yellow
- Red
Correct Option: A
Resolving power or limit of resolution is the ability of the eye to distinguish two close objects as separate. Its value is calculated by Abbe’s equation,
| Lm = | ||
| NA |
where λ is the wavelength of light.
NA or numerical apperture is multiple of refractive index and sine of angle subtended by optical axis and outer ray covered by objective (sina).
