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Match the entries in the Group I with the elution conditions in Group II.
Group I
P.Ion-exchange
Q.Hydrophobic chromatography
R.Gel filtration column chromatography
S.Chromatofocusing
Group II
1.Isocratic solvent chromatography
2.Ampholytes
3.Increasing gradient of salt
4.Decreasing gradient of polarity
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- P-4,Q-1,R-2,S-3
- P-4,Q-3,R-1,S-2
- P-3,Q-4,R-1,S-2
- P-3,Q-4,R-2,S-1
Correct Option: C
In Ion exchange chromatography, be it cation based or anion based resin used, the elution of the substrate bound to the resins is done through increasing concentrations of salt in a stepwise gradient form of elution. For Hydrophobic column chromatography, ligands bound to the column through various interactions can be eluted by decreasing the level of polarity in the solution. In Gel filtration chromatography, molecules elute based on their different molecular weights through the process of sieving from the column by the use of any kind of isocratic solution of particular pH. In Chromatofocusing or also known as Isoelectric focussing, samples of proteins are precipitated at the isoelectric point using ampholytes of different charges.
