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Fed batch cultivation is suitable for which of the following?
P. Processes with substrate inhibition
Q. Processes with product inhibition
R. High cell density cultivation
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- P and Q only
- P and R only
- Q and R only
- P, Q and R
Correct Option: B
A fed-batch culture is a semi-batch operation in which the nutrients necessary for cell growth and product formation are fed either intermittently or continuously via one or more feed streams during the course of an otherwise batch operation. The types of bioprocesses for which fed-batch culture is effective can be summarized as follows :
1.Substrate inhibition,
2.High cell density (High cell concentration)
3.Glucose effect (Crabtree effect)
4.Catabolite repression
5.Auxotrophic mutants
6. Expression control of a gene who has repressible promoter in recombinant cells
7.Extension of operation time, supplement of water lost by evaporation, and decreasing viscosity of culture broth.
