Calculate the percentage sequence identity for the pairwise

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Calculate the percentage sequence identity for the pairwise alignment given below._____
H E L L O –
Y E L L O W

1. 33.5 to 44.7
2. 66.5 to 66.7
3. 22.5 to 33.7
4. 99.5 to 99.7

Correct Option: B

Given, two sequences HELLO- and YELLOW
The % identity between the two sequences would be : No. of identical positions / Total no. of positions
No. of identical positions = 4 (ELLO); Total no. of positions = 6
Therefore, percentage identity = 4 / 6 × 100 = 66.667 %