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Biochemical Miscellaneous

  1. Determine the correctness or otherwise of the following Assertion (A) and the Reason (R). ​​
    Assertion : In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously. ​​
    Reason : Synchronous culture could be obtained by starving cells for essential nutrient components. ​​
    1. ​both (A) and (R) are true and (R) is the correct reason for (A)
    2. both (A) and (R) are true but (R) is not the correct reason for (A) ​​
    3. (A) is true but (R) is false
    4. (A) is false but (R) is true ​
Correct Option: B

Synchronous growth of a bacterial population is that during which all bacterial cells of the population are physiologically identical and in the same stage of cell division cycle at a given time. Synchronous growth helps studying particular stages or the cell division cycle and their interrelations. A synchronous culture can be obtained either by manipulating environmental conditions such as by repeatedly changing the temperature or by adding fresh nutrients to cultures as soon as they enter the stationary phase, or by physical separation of cells by centrifugation or filtration. Synchronous cultures rapidly lose synchrony because not all cells in the population divide at exactly the same size, age or time. ​



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