To optimize the bioreactor system, which one of the following

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To optimize the bioreactor system, which one of the following

To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation ?

1. culture agitation to maintain oxygen supply
2. restriction of the entry of contaminating organisms
3. control of parameters like pH and temperature
4. maintenance of constant culture volume

anerobic fermentation doesn’t need agitation to maintain oxygen supply.