Direction: A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4 × 103kb) genomic library.
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The minimum number of independent recombinant clones required to represent this fragment in genomic library are
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- 16 × 102
- 12 × 102
- 8 × 102
- 1.25 × 102
Correct Option: C
Given, size of the genome of E. coli = 4 × 103kb = 4 × 106 bp
Size of the DNA fragment = 5000 bp
Therefore, minimum number of independent recombinant clones required to represent the DNA fragment in a genomic library = (4 × 106) / 5000 = 8 × 102
