-
The basis for blue-white screening with pUC vectors is
-
- intraallelic complementation
- intergenic complementation
- intragenic suppression
- extragenic suppression
Correct Option: A
Both pUC and the bacterial own genome produce a faulty gene product of Lac Z gene. The lac Z fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic complementation with a defective form of β-galactosidase enzyme encoded by host chromosome (mutation lacZDMI5)