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Identify the CORRECT statements
P. 5′ and 3′ ends of the transcripts can be mapped by utilizing polymerase chain reaction
Q. S1 nuclease can cleave the DNA strand of a DNA-RNA hybrid
R. T4 polynucleotide kinase is used for labeling 3′ end of DNA
S. Baculovirus (Autographa californica) can be used as an insect expression vector
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- P and Q only
- R and S only
- P and S only
- Q and R only
Correct Option: C
Rapid amplification of cDNA ends (RACE) polymerase chain reactions (PCR) are used for independent high-throughput verification of transcriptional starting sites (TSSs) determined by genome-wide assays. 52 -RACE PCR is a well-established and widely used method to specifically amplify the 52 end of a transcript, facilitating mapping of the TSS and the approximate location of promoter elements. Conventionally, this mapping is done by cloning the 52 -RACE PCR product into a bacterial vector and sequencing a few clones by classic electrophoresis-based Sanger sequencing. Now-a-days, even 3’ end transcripts are being mapped using 3’-RACE PCR. Baculoviruses are a very diverse group of viruses with double-stranded, circular, supercoiled genomes, with sizes varying from about 80 to over 180 kb which encode between 90 and 180 genes. Baculoviruses have evolved to initiate infection in the insect midgut. Baculovirus-insect cell expression systems have the capacity to produce many recombinant proteins at high levels and they also provide significant eukaryotic protein processing capabilities.
