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Advanced Biotech Miscellaneous

  1. Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) ​​
    Assertion : Somatic embryogenesis  in plants is a two step process comprising of embryo initiation followed by embryo production. ​​
    Reason : Embryo initiation is independent of the presence of 2, 4 dichlorop-henoxyacetic acid whereas embryo production requires a high concentration of 2, 4-dichloroph-enoxyacetic acid.
    1. both (a) and (r) are true and (r) is the correct reason for (a)
    2. ​both (a) and (r) are true and (r) is not the correct reason for (a) ​​
    3. (a) is true but (r) is false
    4. (a) is false but (r) is true
Correct Option: C

Somatic embryogenesis is a process where a plant or embryo is derived from a single somatic cell or group of somatic cells. Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i.e. ordinary plant tissue. In plants, somatic embryogenesis comprises of embryo initiation followed by embryo production. ​​
Embryo initiation occurs on a medium rich in auxin, which induces differentiation of localized meristematic cells. The auxin typically used is 2,4-Dichlorophenoxyacetic acid (2,4-D). thus embryo initiation is dependent on 2,4-D. While these are transferred to a medium containing low or no auxin, then embryo production does not require high concentration of 2, 4-D. ​



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