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Advanced Biotech Miscellaneous

  1. Match the items in Group I with Group II ​​
    Group–I​  ​​
    (Vectors)
    Group–II
    ​​(Maximum DNA ​​​​​packaging)
    P.​ λ phage​ 1.​ 35–45 kb
    ​​​​Q.​ Bacterial Artificial Chromosomes (BACs) 2.​ 100–300 kb
    R.​ P1 derived Artificial Chromosomes (PACs) ​ 3.​ ≤ 300 kb ​​​
    ​​S. ​λ cosmid​ 4.​ 5–25 kb ​ ​
    ​​ ​​​
    1. P-3, Q-4, R-1, S-2 ​
    2. P-1, Q-3, R-2, S-4
    3. P-4, Q-3, R-2, S-1 ​
    4. P-1, Q-2, R-3, S-4
Correct Option: C

The cloning sites of the vectors are following: Lambda phage has size of 5-24kb, BACs have size of 5300kb, PACS have size of 100-300kb and Cosmids have size of 35-45kb.



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