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Match the items in Group 1 with correct options in Group 2 :
Group 1 Group 2 P. DNA footprinting 1. Protein-protein interaction Q. Yeast two-hybrid 2. VNTR system R. DNA fingerprinting 3. DNA binding protein S. SAGE 4. Transcriptome analysis
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- P-1, Q-2, R-4, S-3
- P-3, Q-1, R-2, S-4
- P-3, Q-4, R-1, S-2
- P-4, Q-2, R-1, S-3
Correct Option: B
⚈ DNA footprinting is a method of investigating the sequence specificity of DNA-binding proteins in vitro. This technique can be used to study protein-DNA interactions both outside and within cells.
⚈ Yeast two-hybrid is based on the reconstitution of a functional transcription factor (TF) when two proteins or polypeptides of interest interact.
⚈ VNTRs have become essential to forensic crime investigations, via DNA fingerprinting and the CODIS database. When removed from surrounding DNA by the PCR or RFLP methods, and their size determined by gel electrophoresis or Southern blotting, they produce a pattern of bands unique to each individual. When tested with a group of independent VNTR markers, the likelihood of two unrelated individuals having the same allelic pattern is extremely improbable.
⚈ Serial analysis of gene expression (SAGE) is a technique used by molecular biologists to produce a snapshot of the messenger RNA population in a sample of interest in the form of small tags that correspond to fragments of those transcripts. It has been successfully used to describe the transcriptome of other diseases and in a wide variety of organisms.
