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A RNA polymerization assay was performed using 3H UTP as the labelled nucleotide with a specific activity of 500 µCi/ µmol (1 µCi = 2.2 × 106 counts per min). After 10 min incubation, the trichloroacetic acid-insoluble radioactivity was found to be 692521 counts per min as determined in a liquid scintillation counter working at 60% efficiency for 3H. The amount of UTP incorporated into the RNA will be
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- 15 nmol
- 105 nmol
- 150 nmol
- 50 nmol
- None of the above
Correct Option: E
Since 60% → 692551 cpm
So, 100% = 1154201.67% cpm
| Number of moles of 3H UTP = | ||
| 500 × 2.2 × 106 cpm |
= 1050 × 10–12 mole
= 1.05 nmole
There is some error in options.
