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To be a cloning vector, a plasmid does NOT require

1. an origin of replication
2. an antibiotic resistance marker
3. a restriction site
4. to have a high copy number

Correct Option: B

for a cloning vector, origin of replication, a restriction site, high copy number and a selectable marker are required. A selectable marker can be of any type not necessarily to be an antibiotic resistance site.