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For protoplast fusion to be successful in plant cells
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- fusion agents other than polyethylene glycol should be used
- cell wall of the two strains of cells should not be damaged
- DNA between the two cells should be compatible
- osmolarity of the medium is not important
Correct Option: C
Protoplasts can be obtained routinely from many plant species, bacteria, yeasts and filamentous fungi. Protoplasts from different strains can sometimes be persuaded to fuse and so overcome the natural sexual mating barriers. However, the range of protoplast fusions is severely limited by the need for DNA compatibility between the strains concerned. Fusion of protoplasts can be enhanced by treatment with the chemical polyethylene glycol, which, under optimum conditions, can lead to extremely high frequencies of recombinant formation which can be increased still further by ultraviolet irradiation of the parental protoplast preparations. Protoplast fusion can also occur with human or animal cell types.
