Direction: Dr. Singh isolated a new 5-kb gene and wants to determine its sequence using a sequencer that can sequence upto 500 bases in a single reaction. Therefore, she decides to create subclones having suitable-size inserts for sequencing
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To generate the minimum number of subclones needed for sequencing, what should be the size of the insert in these subclones ?
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- 1000 bp
- 500 bp
- 250 bp
- 2000 bp
Correct Option: B
insert size should be 500bp
