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Advanced Biotech Miscellaneous

  1. ​The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco 52I ?
    1. EcoRI (G/AATTC) ​​
    2. XmaIII (C/GGCGG) ​​
    3. ​SmaI (CCC/GGG)
    4. SacII (CCGC/GG) ​
Correct Option: B

Check for cutting site specification. Cut by XmaIII can be cloned into a plasmid with Eco521.



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