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The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco 52I ?
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- EcoRI (G/AATTC)
- XmaIII (C/GGCGG)
- SmaI (CCC/GGG)
- SacII (CCGC/GG)
Correct Option: B
Check for cutting site specification. Cut by XmaIII can be cloned into a plasmid with Eco521.