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What is the primary purpose of neomycin in creating mice with knock-outs in gene X?
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- neomycin selects for the survival of embryonic stem cells (ES) that have incorporated the mutant gene X anywhere in the genome
- neomycin selects for the survival of ES cells that have incorporated the mutant gene in the place of the wild-type gene
- neomycin prevents Candida infection during ES cell culture that does not have gene X
- neomycin makes the gene X knock - out mice resistant to Candida infection
Correct Option: A
Common approach to inactivate a selected gene is to insert an antibiotic resistance gene into the cloned gene of interest. This both inactivates the gene of interest and allows identification of ES cells that have been successfully transfected. In the engineered insertion construct, the target gene is disrupted with the neoR gene which confers neomycin resistance.
