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T4 Polynucleotide kinase is used for
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- Labelling 3′ ends of DNA
- Labelling 5′ ends of DNA
- Creating blunt ends of DNA
- Dephosphorylation of DNA
Correct Option: B
T4 Polynucleotide Kinase catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´-hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3´-monophosphates. This modified version exhibits full kinase activity with no 3´ phosphatase activity.
Highlights of T4 Polynucleotide Kinase :
(1) 5´ phosphorylation of DNA/RNA for subsequent ligation
(2) End-labeling of DNA or RNA
(3) 5´ phosphorylation of 3´ phosphorylated mononucleotides to generate a substrate (pNp) that can be added to the 3´ end of DNA or RNA by ligase activity
(4)5´ end labeling of 3´ phosphorylated oligos
